| 1 | Title |
| 2 | Overground (Main Layer) |
| 3 | Jingle - Hexcavators |
| 4 | Spore Judgment (Mushroom Mines) |
| 5 | Boss - The Studious |
| 6 | Boss Defeat |
| 7 | Thermal Vent (Lost Atlantis) |
| 8 | Boss - The Quenched |
| 9 | An Ambush! |
| 10 | Fully Furnaced (Smelt Works) |
| 11 | Boss - The Forger |
| 12 | Jingle - Well Met, Friend! |
| 13 | Overground (Campfire Layer) |
| 14 | One Person's Trash (Magic Landfill) |
| 15 | Boss - The Packrat |
| 16 | Pest Friends (Insect Hive) |
| 17 | Boss - The Chitinous |
| 18 | Jingle - Treasure! |
| 19 | Overground (Hoofman's Shop Layer) |
| 20 | Beware the Depths |
| 21 | Who Needs Honor (Drill Knight's Castle) |
| 22 | Boss - The Claim Jumper |
| 23 | Hegemony (Crystal Core) |
| 24 | Boss - The Awakening |
| 25 | Rapid Ascent |
| 26 | Score Screen |
| 27 | End Credits |
| 28 | Epilogue |
| 29 | Trailer (Date Reveal) |
